MP72-20 CD31 ANTIBODY CONJUGATION IMPROVES RE-ENDOTHELIALIZATION OF ACELLULAR KIDNEY SCAFFOLDS FOR WHOLE KIDNEY ENGINEERING

Abstract

You have accessJournal of UrologyTransplantation & Vascular Surgery II1 Apr 2014MP72-20 CD31 ANTIBODY CONJUGATION IMPROVES RE-ENDOTHELIALIZATION OF ACELLULAR KIDNEY SCAFFOLDS FOR WHOLE KIDNEY ENGINEERING In Kap Ko, Mehran Abolbashari, Jennifer Huling, Joao Zambon, Cheil Kim, John Jackson, Delrae Eckman, Anthony Atala, and James Yoo In Kap KoIn Kap Ko More articles by this author , Mehran AbolbashariMehran Abolbashari More articles by this author , Jennifer HulingJennifer Huling More articles by this author , Joao ZambonJoao Zambon More articles by this author , Cheil KimCheil Kim More articles by this author , John JacksonJohn Jackson More articles by this author , Delrae EckmanDelrae Eckman More articles by this author , Anthony AtalaAnthony Atala More articles by this author , and James YooJames Yoo More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.2259AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The lack of transplantable allografts for renal failure has prompted the development of scaffold design and cell isolation techniques to re-create an engineered whole kidney. Decellularized solid organ xenografts such as the kidney hold great promise due to the preservation of structural integrity while removing all cellular components. However, acellular kidney vasculature needs an endothelial cell lining to avoid blood clotting and thrombus formation when transplanted into a living recipient. We developed a surface modification method to reinforce endothelial cell attachment onto renal vasculature via antibody conjugation, and evaluated the effectiveness of re-endothelialization in vivo through transplantation. METHODS Endothelial cell specific antibodies (CD31) were conjugated on the collagen pre-coated surfaces of microfluidic flow chamber to determine the levels of endothelial (MS1) cell attachment under various flow rates (20 to 100ml/hr). Decellularized porcine kidney scaffolds conjugated with CD31 antibodies were seeded with MS1 cells and preconditioned using a perfusion bioreactor. The re-endothelialized kidney scaffolds were transplanted in pigs. Angiographic and histological examinations were performed to determine the intactness of vascular trees, re-endothelialization of vasculatures, and platelet adhesion on vasculatures within the renal scaffold. RESULTS At all flow rates tested, there was no measurable detachment of MS1 cells in the antibody conjugated flow chambers. Angiography of the implanted kidneys showed improved vascular patency in all vessel sizes from the antibody treated scaffolds, as compared to the untreated scaffolds. This functional outcome was supported by the results of histological studies showing that CD31 antibody conjugation maintained the endothelialization of intermediate- and small- sized vasculatures, as well as preventing platelet adhesion. CONCLUSIONS Antibody conjugation-mediated MS1 cell attachment on the vasculature of implanted scaffolds improved vascular patency throughout the renal parenchyma. This functional outcome is supported by the maintenance of MS1 attachment and prevention of platelet adhesion on the endothelialized vasculatures during implantation. These results indicate that antibody conjugation improves re-endothelialization of vascular trees of bioengineered tissue, and this method could be used to engineer whole kidney. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e831 Advertisement Copyright & Permissions© 2014MetricsAuthor Information In Kap Ko More articles by this author Mehran Abolbashari More articles by this author Jennifer Huling More articles by this author Joao Zambon More articles by this author Cheil Kim More articles by this author John Jackson More articles by this author Delrae Eckman More articles by this author Anthony Atala More articles by this author James Yoo More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...