Abstract
Epidermal keratinocytes are continuously dividing cells which, upon differentiation establish the outer skin barrier, the stratum corneum. In older age the epidermis becomes thinner, papillary protrusions flatten and the capacity for skin barrier repair decreases. Here we performed single-cell RNA sequencing of epidermal keratinocytes obtained by suction blisters from the volar forearm of younger (20-30) and older (65-70) healthy female individuals. Based on the expression of keratin (KRT) 5 and 10 and other structural proteins including filaggrin and corneodesmosin, we detected nine keratinocyte clusters at distinct differentiation stages amongst others.
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