Abstract
The objective of this study was to investigate the distribution of carbohydrate in the mandibular and lingual glands of layer in difference age groups histochemically.The study was observed the mandibular and lingual glands from nine layers which grouped into three groups namely starter aged 40 days old, productive aged seven months old, and post-productive aged 1.5 years old. Each group consisted of three layers. A small piece of mandibular and lingual glands were fixed in Bouin solution for subsequent processed into histological preparation.The distribution of carbohydrate in both of glands detected by alcian blue staining (AB) pH 2.5 and periodic acid schiff (PAS). The result showed that mandibular and lingual glands of the three group layers reacted positively to AB pH 2.5 and PAS which found in the cytoplasm of cell and in the secretion on lumen. In the cytoplasm, the mandibular and lingualis reacted positively to AB pH 2.5 and PAS with the intensity ranging from weak reaction(+) to strong (+++), while in the lumen of glands showed negative reaction (-) to positive with the intensity of weak (+) to strong (+++). In mandibular gland, the acid carbohydrates were found with strong intensity (+++) in the productive and post-productive layer, while neutral carbohydrates were found with the same intensity on starter, productive and post-productive layers. In the lingual gland, the acid carbohydrates found with strong intensity in the three groups of layers while the neutral were found with strong intensity (+++) in the starter and post-productive layer. Positive reaction to AB pH 2.5 and PAS indicated that both of the salivary glands contain the neutral and acid carbohydrate. ____________________________________________________________________________________________________________________ Key words: mandibular gland, lingual gland, acid carbohydrate, neutral carbohydrate, layer
Highlights
The objective of this study was to investigate the distribution of carbohydrate
lingual glands from nine layers which grouped into three groups
1.5 years old. Each group consisted of three layers
Summary
Vol 10 No 2, Mei 2016 metode pewarnaan alcian blue (AB) untuk karbohidrat asam dan pewarnaan periodic acid Schiff (PAS) untuk karbohidrat netral. Kajian histokimia sebaran karbohidrat pada kelenjar saliva telah dilaporkan pada beberapa spesies hewan, diantaranya tupai (Tupaia glis) (Zainuddinet al., 2000), ayam broiler (Gallus sp.) dan burung puyuh (Cortunix cortunix) (Adnyaneet al., 2007), kambing, kucing, dan babi (Adnyane, 2009), serta burung walet linchi (Collocalia linchi) (Novelina et al, 2010). Menurut Adnyane et al (2007), kandungan karbohidrat pada sitoplasma sel dan sekreta dari kelenjar mandibularis dan kelenjar lingualis pada ayam broiler umur 28 hari mengandung karbohidrat asam dan karbohidrat netral dengan intensitas lemah sampai kuat. Penelitian ini bertujuan mengetahui sebaran karbohidrat pada kelenjar mandibularis dan kelenjar lingualis ayam secara histokimia pada kelompok umur ayam petelur yang berbeda. Penelitian ini diharapkan menjadi informasi dasar tentang pola sebaran karbohidrat pada kelenjar mandibularis dan kelenjar lingualis ayam petelur dengan umur yang berbeda, serta sebagai referensi bagi penelitian selanjutnya
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