2087 OXYGEN NANO-BUBBLE WATER EXERTS INHIBITORY EFFECTS ON KIDNEY STONE FORMATION BY PROTECTING RENAL TUBULAR CELL INJURY

Abstract

You have accessJournal of UrologyStone Disease: Basic Research (I)1 Apr 20132087 OXYGEN NANO-BUBBLE WATER EXERTS INHIBITORY EFFECTS ON KIDNEY STONE FORMATION BY PROTECTING RENAL TUBULAR CELL INJURY Yasuhiko Hirose, Takahiro Yasui, Kazumi Taguchi, Kazuhiro Niimi, Shuzo Hamamoto, Atsushi Okada, Yasunori Ito, Shoichi Sasaki, and Kenjiro Kohri Yasuhiko HiroseYasuhiko Hirose Nagoya, Japan More articles by this author , Takahiro YasuiTakahiro Yasui Nagoya, Japan More articles by this author , Kazumi TaguchiKazumi Taguchi Nagoya, Japan More articles by this author , Kazuhiro NiimiKazuhiro Niimi Nagoya, Japan More articles by this author , Shuzo HamamotoShuzo Hamamoto Nagoya, Japan More articles by this author , Atsushi OkadaAtsushi Okada Nagoya, Japan More articles by this author , Yasunori ItoYasunori Ito Nagoya, Japan More articles by this author , Shoichi SasakiShoichi Sasaki Nagoya, Japan More articles by this author , and Kenjiro KohriKenjiro Kohri Nagoya, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.2506AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Renal tubular cell injury induced by CaOx crystals plays an important role in kidney stone formation. Water containing oxygen nano-bubbles (ONB: nanometer-sized bubbles generated from oxygen micro-bubbles) has anti-inflammatory effects. Therefore, we investigated the inhibitory effects of ONB water on kidney stone formation in ethylene glycol (EG)-treated rats. METHODS ONB water can be produced by rapidly collapsing oxygen micro-bubbles in hard water with a salt concentration of 1.0% by mass. Further, ONB water with a salt concentration of 0% by mass can be prepared by passing the resulting ONB water twice through a reverse osmosis membrane, obtaining ONB water was defined as 100% ONB water. A mixture comprising half 100% ONB water and half distilled water was defined as 50% ONB water. We divided 60 rats, aged 4 weeks, into 5 groups: (1) control, a water-fed group; (2) 100% ONB, a 100% ONB water-fed group; (3) EG, the EG-treated water-fed group; and (4), and (5) EG+50% ONB and EG+100% ONB, the 2 EG-treated, ONB water-fed groups. The rats consumed these fluids by free access for 5 weeks, and the EG-treated groups were also administered 1.2 mL of 10% EG and 0.5 μg of vitamin D3 via a stomach tube for the last 2 weeks. Six rats in each group were sacrificed after 7 days and 6 after 14 days. The pooled 24-hour urine and blood samples of these rats were collected, and their kidneys were excised. Renal CaOx deposits, urinary excretion of N-acetyl-β-D-glucosaminidase (NAG), and renal expression of MCP-1 and OPN as inflammation-related proteins, SOD-1 as an oxidative stress biomarker, and the crystal binding molecule hyaluronic acid were compared among the 5 groups. RESULTS In the EG group, CaOx deposits tended to increase at 14 days compared to 7 days after EG administration. However, CaOx deposits were significantly lower in the EG+50% ONB and EG+100% ONB groups than in the EG group 14 days after administration (Figure). In the EG+50% ONB and EG+100% ONB groups, ONB water significantly decreased urinary NAG excretion, and renal MCP-1, OPN, and hyaluronic acid expression, and increased renal SOD-1 expression compared to the EG group. CONCLUSIONS ONB water exerted substantial effects on kidney stone formation in the rat kidney by protecting renal tubular cell injury. ONB water may be a prophylactic for kidney stones. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e856 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yasuhiko Hirose Nagoya, Japan More articles by this author Takahiro Yasui Nagoya, Japan More articles by this author Kazumi Taguchi Nagoya, Japan More articles by this author Kazuhiro Niimi Nagoya, Japan More articles by this author Shuzo Hamamoto Nagoya, Japan More articles by this author Atsushi Okada Nagoya, Japan More articles by this author Yasunori Ito Nagoya, Japan More articles by this author Shoichi Sasaki Nagoya, Japan More articles by this author Kenjiro Kohri Nagoya, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...