MP20-13 OXYGEN NANO-BUBBLE WATER EXERTS INHIBITORY EFFECTS ON KIDNEY STONE FORMATION BY INHIBITING RENAL TUBULAR CELL INJURY

Abstract

You have accessJournal of UrologyStone Disease: Basic Research I1 Apr 2014MP20-13 OXYGEN NANO-BUBBLE WATER EXERTS INHIBITORY EFFECTS ON KIDNEY STONE FORMATION BY INHIBITING RENAL TUBULAR CELL INJURY Yasuhiko Hirose, Kazumi Taguchi, Yasuhiro Fujii, Kazuhiro Niimi, Shuzo Hamamoto, Ryosuke Ando, Masahito Hirose, Atsushi Okada, Yasue Kubota, Yasunori Ito, Takahiro Yasui, Keiichi Tozawa, and Kenjiro Kohri Yasuhiko HiroseYasuhiko Hirose More articles by this author , Kazumi TaguchiKazumi Taguchi More articles by this author , Yasuhiro FujiiYasuhiro Fujii More articles by this author , Kazuhiro NiimiKazuhiro Niimi More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Ryosuke AndoRyosuke Ando More articles by this author , Masahito HiroseMasahito Hirose More articles by this author , Atsushi OkadaAtsushi Okada More articles by this author , Yasue KubotaYasue Kubota More articles by this author , Yasunori ItoYasunori Ito More articles by this author , Takahiro YasuiTakahiro Yasui More articles by this author , Keiichi TozawaKeiichi Tozawa More articles by this author , and Kenjiro KohriKenjiro Kohri More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.735AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Renal tubular cell injury induced by oxalate plays an important role in kidney stone formation. Water containing oxygen nano-bubbles (ONB: nanometer-sized bubbles generated from oxygen micro-bubbles) has anti-inflammatory effects. Therefore, we investigated the inhibitory effects of ONB water on kidney stone formation in ethylene glycol (EG)-treated rats. METHODS ONB water can be produced by rapidly collapsing oxygen micro-bubbles in hard water with a salt concentration of 1.0% by mass. Further, ONB water with a salt concentration of 0% by mass can be prepared by passing the resulting ONB water twice through a reverse osmosis membrane, obtaining ONB water was defined as 100% ONB water. A mixture comprising half 100% ONB water and half distilled water was defined as 50% ONB water. We divided 60 rats, aged 4 weeks, into 5 groups: (1) control, a water-fed group; (2) 100% ONB, a 100% ONB water-fed group; (3) EG, the EG-treated water-fed group; and (4), and (5) EG+50% ONB and EG+100% ONB, the 2 EG-treated, ONB water-fed groups. The rats consumed these fluids by free access for 5 weeks, and the EG-treated groups were also administered 1.2 mL of 10% EG and 0.5 μg of vitamin D3 via a stomach tube for the last 2 weeks. Six rats in each group were sacrificed after 7 days and 6 after 14 days. The pooled 24-hour urine and blood samples of these rats were collected, and their kidneys were excised. Renal CaOx deposits, urinary excretion of N-acetyl-β-D-glucosaminidase (NAG), and renal expression of MCP-1 and OPN as inflammation-related proteins, and the crystal binding molecule hyaluronic acid were compared among the 5 groups. RESULTS In the EG group, CaOx deposits tended to increase at 14 days compared to 7 days after EG administration. However, CaOx deposits were significantly lower in the EG+50% ONB and EG+100% ONB groups than in the EG group 14 days after administration (Figure). In the EG+50% ONB and EG+100% ONB groups, ONB water significantly decreased urinary NAG excretion, and renal MCP-1, OPN, and hyaluronic acid expression compared to the EG group. CONCLUSIONS ONB water exerted substantial effects on kidney stone formation in the rat kidney by inhibiting renal tubular cell injury. ONB water may be a prophylactic for kidney stones. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e201 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Yasuhiko Hirose More articles by this author Kazumi Taguchi More articles by this author Yasuhiro Fujii More articles by this author Kazuhiro Niimi More articles by this author Shuzo Hamamoto More articles by this author Ryosuke Ando More articles by this author Masahito Hirose More articles by this author Atsushi Okada More articles by this author Yasue Kubota More articles by this author Yasunori Ito More articles by this author Takahiro Yasui More articles by this author Keiichi Tozawa More articles by this author Kenjiro Kohri More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...