2050 HYDROXYPROLINE AND GELATIN DIETS INDUCE LONG-TERM HYPEROXALURIA IN THE PORCINE MODEL

Abstract

You have accessJournal of UrologyStone Disease: Basic Research1 Apr 20112050 HYDROXYPROLINE AND GELATIN DIETS INDUCE LONG-TERM HYPEROXALURIA IN THE PORCINE MODEL Sutchin R. Patel, Kristina L. Penniston, Lauren Iwicki, Ibrahim Saeed, Thomas D. Crenshaw, and Stephen Y. Nakada Sutchin R. PatelSutchin R. Patel Madison, WI More articles by this author , Kristina L. PennistonKristina L. Penniston Madison, WI More articles by this author , Lauren IwickiLauren Iwicki Madison, WI More articles by this author , Ibrahim SaeedIbrahim Saeed Madison, WI More articles by this author , Thomas D. CrenshawThomas D. Crenshaw Madison, WI More articles by this author , and Stephen Y. NakadaStephen Y. Nakada Madison, WI More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.2281AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Swine models have been useful in the study of many different disease processes, but the anatomic and physiologic renal similarities between sows and humans make them a promising model for the study of nephrolithiasis. Previous porcine research has shown that a 5% hydroxyproline diet can lead to short-term induction of hyperoxaluria with a decrease in urinary oxalate by day 5. A gelatin diet has been shown in humans to induce hyperoxaluria. The purpose of our study was to determine if a hydroxyproline or gelatin diet could induce long-term hyperoxaluria in the porcine model. METHODS A total of 9 gravid crossbred sows (Large White x Landrace) were randomly allotted into 3 treatment groups: 5% Hydroxyproline, 10% Hydroxyproline and Gelatin diet. All sows were catheterized one day prior to starting treatment diet. Catheters were left in place for 5 days prior to being removed. Sows were recatheterized for urine collection on day 21. Urine was collected for each entire 24 hour period in order to control for differences in the diurnal and postprandial variations in the renal handling of oxalate. Urinary oxalate was determined by ion chromatography. RESULTS In the 5% hydroxyproline diet, the percentage increase in urinary oxalate from baseline peaked to 133% on day 2 and was 288% on day 21. In the 10% hydroxyproline diet, the percentage increase in urinary oxalate from baseline peaked to 312% on day 3 and was 322% on day 21. In the gelatin diet, the percentage increase in urinary oxalate from baseline peaked to 331% on day 3 and was 317% on day 21. Paired Student's t-test confirmed that oxalate levels were significantly different from baseline in the 5% hydroxyproline diet on day 21 (p<0.001) and in the gelatin diet on days 3 and 21 (p=0.02 and p=0.03). To further confirm that the treatment diets had the expected impact on urinary oxalate, mass spectrometry was used to confirm increased urinary glycolate in a subset of the samples. CONCLUSIONS All three treatment diets lead to the induction of long-term hyperoxaluria in a porcine model. The gelatin diet induced statistically significant hyperoxaluria over the greatest number of time points among the three treatment groups. The ability to induce long-term hyperoxaluria has important implications in establishing a porcine model for calcium oxalate urolithiasis. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e820-e821 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sutchin R. Patel Madison, WI More articles by this author Kristina L. Penniston Madison, WI More articles by this author Lauren Iwicki Madison, WI More articles by this author Ibrahim Saeed Madison, WI More articles by this author Thomas D. Crenshaw Madison, WI More articles by this author Stephen Y. Nakada Madison, WI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...