2073 DIETARY HYDROXYPROLINE INDUCES NEPHROLITHIASIS IN THE ADULT PORCINE MODEL

Abstract

You have accessJournal of UrologyStone Disease: Basic Research I1 Apr 20122073 DIETARY HYDROXYPROLINE INDUCES NEPHROLITHIASIS IN THE ADULT PORCINE MODEL Sutchin R. Patel, Kristina L. Penniston, Thomas D. Crenshaw, and Stephen Y. Nakada Sutchin R. PatelSutchin R. Patel Lake Bluff, IL More articles by this author , Kristina L. PennistonKristina L. Penniston Madison, WI More articles by this author , Thomas D. CrenshawThomas D. Crenshaw Madison, WI More articles by this author , and Stephen Y. NakadaStephen Y. Nakada Madison, WI More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.2239AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Our previous studies have shown that a 10% hydroxyproline and gelatin diet can both lead to long-term hyperoxaluria in the adult porcine model. However, no studies have shown nephrolithiasis formation in the adult porcine model. The purpose of our study was to evaluate both diets in the sow model for histologic crystal deposition and gross formation of nephrolithiasis. METHODS Two sows were fed a control diet and six sows were fed a diet of either 10% hydroxyproline or gelatin for 7, 14 and 21 days. Catheters were placed in the sows for 24 hour urine collection prior to the sows being sacrificed at either the 7 day, 14 day or 21 day timepoint. Both kidneys were removed and evaluated for gross stone formation. Renal tissue was fixed in formalin, stained with H&E and von Kossa and evaluated under light microscopy for calcium crystal deposition. Urine was analyzed via ion chromatography for oxalate. RESULTS Mean urinary oxalate concentration for sows on the control diet was 74 mg/L. Urinary oxalate concentrations for the 10% HP diet were 147, 352 and 89 mg/L at 7, 14 and 21 days. Urinary oxalate concentrations for the gelatin diet were 46, 4 and 132 mg/L at 7, 14 and 21 days. Histology revealed calcium crystal formation in the distal collecting ducts and renal papillae in one kidney of the 21 day, 10% hydroxyproline diet sow. Gross pathology revealed 7 renal calculi and calcification at the papillary tips in the same kidney of the 21 day, 10% hydroxyproline diet sow. Chemical analysis of the calculi revealed a composition of 20% calcium oxalate monohydrate, 78% calcium oxalate dihydrate and 2% protein. CONCLUSIONS A long-term 10% hydroxyproline diet leads to calcium oxalate nephrolithiasis in the adult porcine model. This is the first reported case of calcium stone formation in the adult porcine model and has important implications in the development of a dietary porcine model for urolithiasis. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e836 Peer Review Report Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sutchin R. Patel Lake Bluff, IL More articles by this author Kristina L. Penniston Madison, WI More articles by this author Thomas D. Crenshaw Madison, WI More articles by this author Stephen Y. Nakada Madison, WI More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...