Abstract

In this paper, we summarize a global survey of 484 participants of the imaging community, conducted in 2020 through the NIH Center for Open BioImage Analysis (COBA). This 23-question survey covered experience with image analysis, scientific background and demographics, and views and requests from different members of the imaging community. Through open-ended questions we asked the community to provide feedback for the open-source tool developers and tool user groups. The community’s requests for tool developers include general improvement of tool documentation and easy-to-follow tutorials. Respondents encourage tool users to follow the best practices guidelines for imaging and ask their image analysis questions on the Scientific Community Image forum (forum.image.sc). We analyzed the community’s preferred method of learning, based on level of computational proficiency and work description. In general, written step-by-step and video tutorials are preferred methods of learning by the community, followed by interactive webinars and office hours with an expert. There is also enthusiasm for a centralized location online for existing educational resources. The survey results will help the community, especially developers, trainers, and organizations like COBA, decide how to structure and prioritize their efforts.

Highlights

  • Impact statement: The Bioimage analysis community consists of software developers, imaging experts, and users, all with different expertise, scientific background, and computational skill levels

  • Of the 484 survey participants, the majority were from North America (60%) and Europe (34%), followed by Asia, Australia, South America, and Africa (Supplementary Fig 1A). 43% of participants were in training

  • Most participants described experience and/or training in the biological sciences, including cell/molecular biology, chemistry/biochemistry, followed by physics and developmental biology (Figure 1B), though the small proportion of participants whose role was “Image analyst” or “Other” reported computational backgrounds more often. This is consistent with a 2015 survey from the Network of European BioImage Analysts (NEUBIAS) 1, which is a network working toward bridging the efforts between life science, computer science, and digital image processing 2

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Summary

Introduction

Impact statement: The Bioimage analysis community consists of software developers, imaging experts, and users, all with different expertise, scientific background, and computational skill levels. The NIH funded Center for Open Bioimage Analysis (COBA) was launched in 2020 to serve the cell biology community’s growing need for sophisticated open-source software and workflows for light microscopy image analysis. This paper shares the result of a COBA survey to assess the most urgent ongoing needs for software and training in the community and provide a helpful resource for software developers working in this domain. We describe the state of open-source bioimage analysis, developers’ and users’ requests from the community, and our resulting view of common goals that would serve and strengthen the community to advance imaging science

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