Abstract
Creating and controlling the cellular microenvironment is considered integral to maintain long-term physiological phenotype and functional state of the cells. Cellular microenvironment is greatly influenced by its interaction with the substrate and neighboring cells. Therefore for in-depth understanding of such microenvironment, precise positioning and patterning of the cells are needed. With the advancements in the microscale technologies, a number of cell patterning techniques have been explored. Among these techniques, dielectrophoresis (DEP)-based cell patterning is advantageous due to its speed, high resolution, precision, ease of operation, and low degree of cell damage. This chapter presents a comprehensive review of the recent advances in article reviews, the theoretical background of DEP, theoretical models associated with cells influencing dielectrophoretic force, recent advances in DEP-based patterning techniques including patterning buffer, cellular characteristics, electrode design, and two-dimensional and three-dimensional cell patterning.
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