Abstract
Surface patterning becomes an important tool in biomedical research and applications, as it enables systematic investigation of cell-biomaterial interaction as well as rapid, high-throughput tests for disease diagnosis and drug screening. Recent advances in three-dimensional patterning allow for recapitulation of cellular microenvironment, providing valuable insights into the interplay between biomolecules, cells and biomaterials and facilitating the generation of personalized tissues for regenerative medicine applications. In this chapter, we review a diverse set of surface patterning techniques and their applications for biomedical use. First, we present important figures-of-merit of surface patterning techniques that should be considered to identify an appropriate method for a particular application. Especially, we discuss common concerns and requirements that need to be addressed in patterning of biologically active substances. We then review various types of patterning techniques mainly on the working principle and interesting examples for biomedical applications.
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