Abstract
Publisher Summary This chapter discusses the glutamate accumulation into synaptic vesicles. A critical, but rate-limiting step, in the study of glutamate transport into synaptic vesicles is the preparation of synaptic vesicles that are free of contamination yet retain functional capability. However, this requires rather large quantities of affinity-purified antibodies to a synaptic vesicle protein, synapsin I, and, despite this requirement, the yield of such a vesicle preparation is too low to permit the analysis of vesicular glutamate uptake in small tissues. The chapter describes the assay and properties of the glutamate transport system in the synaptic vesicle in three preparations: (1) bovine brain synaptic vesicles highly purified by the use of antisynapsin I immunoglobulin G (IgG), (2) a bovine brain synaptic vesicle fraction purified by sucrose density gradient centrifugation, and (3) a rat brain crude synaptic vesicle fraction. Beyond the characterization of the properties of glutamate uptake into synaptic vesicles, little research has investigated in vivo changes in vesicular uptake.
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