2'-C,4'-C-Ethyleneoxy-Bridged 2'-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability.

Takashi Osawa,Satoshi Obika,Yoshiyuki Hari

doi:10.1007/978-1-4939-9216-4_5

2'-C,4'-C-Ethyleneoxy-Bridged 2'-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability.

Takashi Osawa, Satoshi Obika + Show 1 more

https://doi.org/10.1007/978-1-4939-9216-4_5

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Journal: Methods in molecular biology (Clifton, N.J.)	Publication Date: Jan 1, 2019
Citations: 1

Affiliation: Tokushima Bunri University, Osaka University

#Duplex-Forming Ability #Deoxyribonucleic Acids + Show 5 more

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Abstract

This chapter describes procedures for (1) the synthesis of six 2'-C,4'-C-ethyleneoxy-bridged thymidine phosphoramidites, i.e., methylene-EoDNA-T, (R)-Me-methylene-EoDNA-T, (S)-Me-methylene-EoDNA-T, EoDNA-T, (R)-Me-EoDNA-T, and (S)-Me-EoDNA-T phosphoramidites, (2) the introduction of the phosphoramidites into oligonucleotides, (3) UV-melting experiments of the duplexes of the modified oligonucleotides and complementary RNA, and (4) nuclease degradation experiments of the modified oligonucleotides.

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