2-Amino sugar-containing oligosaccharides isolated from hydrazinolyzate of blood group A mucopolysaccharide of hog gastric mucus. II. Isolation and characterization of O-beta-2-acetamido-2-deoxy-D-glucopyranosyl-(1-6)-D-galactose and O-beta-2-acetamido-2-deoxy-D-glucopyranosyl-(1-3)-[O-beta-2-acetamido-2-deoxy -D-glucopyranosyl-(1-6)]-D-galactose.

Abstract

The large fractions 4 and 5, described in the first report, obtained after the first cellulose powder column chromatography of the N-acetylated 2-amino sugar-containing compounds which were separated from the acid hydrolyzate of the hydrazinolyzed product of blood group A mucopolysaccharide of hog gastric mucus, were further fraction ated on cellulose powder columns and char coal columns. Of the fractions, a disaccharide (Oligo saccharide III) and a trisaccharide (Oligosac charide IV) were isolated. On the basis of the analytical data as well as the values of sodium metaperiodate oxidation before and after reduction with sdyodium borohydride and their physical properties, the structures of these compounds have been firmly established: Oligosaccharide III is O-β-2-acetamido-2-deoxy-D-glucopyranosyl-(1→6)-D-galactose and Oligosaccharide IV is O-β-2-acetamido-2-dexoy-D-glucopyransol-(1→3)-[O-β-2acetamido-2-deoxy-D- glucopyra-(1→6)-]-D-galactose.