Isolation and assessment of acidic and neutral oligosaccharides from goat milk and bovine colostrum for use as ingredients of infant formulae

Abstract

Acidic and neutral oligosaccharides (OSs) from goat milk or bovine colostrum were assessed for the potential uses as ingredients of infant formulae or functional foods. For that purpose, neutral and acidic OS fractions were isolated by a modified charcoal column method. The elution protocol of the charcoal column was improved to decrease the column size to approximately 1/9 of the traditional process. Then the inhibitory potencies of the neutral and acidic OSs isolated from the two mammals were compared at 2.5 and 5.0 mg mL−1 using OSs from human breast milk as a reference. These OS fractions inhibited the adhesion of Salmonella enterica to Caco-2 cells, although some differences were not statistically significant. Finally, goat milk OSs were determined as more potential ingredients of infant formulae or functional foods in the aspect of the potency of adherence inhibition, safety, and the availability of the starting material.