2, 4, 5-TRIHYDROXYBUTYROPHENONE (2, 4, 5-THBP)aAS A SUBSTRATE FOR MUSHROOM TYROSINASE

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2, 4, 5-THBP (2, 4, 5-trihydroxybutyrophenone) is a substrate for mushroom tyrosinase with a Km value of 1.00 mM. The final product(s) formed is red (λ.max 480–490 nm) and it is rather stable with time. An intermediate red product(s), having a maximum at λ 505–515 nm, was detected and the time course of its conversion to the final red product(s) was studied. The data suggest that the intermediate red product(s) is 2, 4, 5-THBP-quinone which is converted to colorless polymerized 2, 4, 5-THBP-OH, which is then oxidized to a red polymerized 2, 4, 5-THBP-quinone(s), which is the final red product(s).