Abstract
Phosphorus is one of the most important constituents of human biofilms, in particular lipid bilayer packing, phase transition (gel phase, physiological liquid crystal phase, ripple phase, non-biphasic), lipid head group orientation/mechanics, and pure lipid bilayers. Phosphorus observations in biofilms play an important role in the study of the interaction of many types of proteins and biofilms in the human body. The design and construction of a 1H-31P double resonance probe with a scroll coil for solid-state NMR experiment are introduced. For good efficiency at the relatively high frequency, minimal RF sample heating during the experiment with a lossy sample, and a wide range of tuning frequency, this probe adapted the low inductance of a scroll coil. The 31P NMR spectra obtained on the biological lossy sample were used to demonstrate the possibility for monitoring the protein dynamics on biomembrane mimetic system and phase change of phospholipid.
Highlights
Phosphorus is one of the most abundant minerals in the human body and is an essential element for life
We introduce the 1H-31P home-built double resonance solid-state Nuclear magnetic resonance (NMR) probe with a scroll coil for 400-MHz NB magnet for lossy biological samples
The photograph of the complete probe assembly is shown in Fig. 1. 31P NMR experimental results obtained by using the 400-MHz 1H- 31P double resonance home-built probe are shown in Figs. 3 and 4
Summary
Phosphorus is one of the most abundant minerals in the human body and is an essential element for life. Phosphorus is a component of phospholipids, one of the components of human biological membrane, and phospholipids form a bilayer in vivo (Marcus 2013). Membrane proteins are present in biological membrane consisting of phospholipid bilayers, and they have a variety of structures and perform various functions essential for survival through the role of ion channels, receptors, and transporters (Cooper 2000). We introduce the 1H-31P home-built double resonance solid-state NMR probe with a scroll coil for 400-MHz NB magnet for lossy biological samples. Since the scroll coil is compatible with a lumped element circuit that is a well-matched transmitter and receiver system at the proper frequency, this resonator is good for a multi-channel SSNMR probe
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call