Abstract
Objective To study the expression of stromal cell derived factor (SDF)-1, chemokine CXC motif receptor(CXCR)-4, matrix metalloproteinase (MMP)-2 and Ki-67 and their association with clinical pathological features in human cervical squamous cancer tissues. Methods From January 2010 to September 2012, a total of 60 cases samples with squamous carcinoma of the cervix which were confirmed by histopathology were enrolled into this study (study group) . At the same time, another 60 cases samples with benign uterine diseases were included into control group. There had no significant differences among age and other clinical information between two groups (P>0.05) . The expression of SDF-1, CXCR4, MMP-2 and Ki-67 were detected between two groups by immunohistochemistry and the correlation analysis was conducted. The study protocol was approved by the Ethical Review Board of Investigation in Second Affiliated Hospital, Medical School of Qingdao University. Informed consent was obtained from all participates . Results The positive expression rates of SDF-1, CXCR4, MMP-2 and Ki-67 were 90.00%, 68.33 %, 70.00% and 86.67% in study group, 40.00%, 8.33%, 13.33% and 1.67% in control group, respectively. Significant differences were observed between two groups (χ2 =9.63, 7.04, 4.00, 4.00; P< 0.05) . Expressions of SDF-1, CXCR4, MMP-2 and Ki-67 in cervical cancer tissues with pelvic lymph nodes metastasis were higher than those in cervical cancer tissues without pelvic lymph node metastasis, which were closely associated with pelvic lymph node metastasis (χ2 =16.692, P<0.001; χ2=8.496, P<0.01; χ2 =4.762, P<0.001; χ2 =6.125, P<0.05) . The expression of SDF-1 was significantly correlated with CXCR4, MMP-2 and Ki-67 (r=0.586, P =0.002; r =0.419, P =0.025; r =0.645, P <0.001) . Conclusions Expressions of SDF-1, CXCR4, MMP-2 and Ki-67 of cervical squamous cancer tissues are involved in tumor genesis and associated with invasion and lymphnode metastasis of cervical cancer, which can serve as biomarkers for diagnosis and prediction of lymph node metastasis. Furthermore, the expression of SDF-1/ CXCR4 in cervical cancer may promote the tumor invasion and metastasis through the secretion of MMP-2 and Ki-67, suggesting that SDF-1 may be an important target for drug therapy. Key words: Uterine cervical neoplasms; Stromal cell derived factor-1; Chemokine CXC motif receptor-4; Matrix metalloproteinases; Ki-67; Immunohistochemistry
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