1968 THE ROLE OF URINARY NEUROTROPHIC FACTORS IN OVERACTIVE BLADDER SYNDROME

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Non-Neurogenic Voiding Dysfunction1 Apr 20121968 THE ROLE OF URINARY NEUROTROPHIC FACTORS IN OVERACTIVE BLADDER SYNDROME Tiago Antunes-Lopes, Rui Pinto, Sérgio Carvalho-Barros, Francisco Botelho, Paulo Diniz, Carlos Martins-Silva, Célia Duarte-Cruz, and Francisco Cruz Tiago Antunes-LopesTiago Antunes-Lopes Porto, Portugal More articles by this author , Rui PintoRui Pinto Porto, Portugal More articles by this author , Sérgio Carvalho-BarrosSérgio Carvalho-Barros Porto, Portugal More articles by this author , Francisco BotelhoFrancisco Botelho Porto, Portugal More articles by this author , Paulo DinizPaulo Diniz Porto, Portugal More articles by this author , Carlos Martins-SilvaCarlos Martins-Silva Porto, Portugal More articles by this author , Célia Duarte-CruzCélia Duarte-Cruz Porto, Portugal More articles by this author , and Francisco CruzFrancisco Cruz Porto, Portugal More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.2127AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Most well known neurotrophic factors (NFs) include nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF). While NGF and BDNF are committed with a peptidergic subpopulation of primary afferents, GDNF is important to maintain the non-peptidergic subpopulation, both in central and peripheral nervous system. The aims of our study were: 1) to investigate urinary levels of NGF, BDNF and GDNF in OAB patients and in healthy controls, 2) to assess urinary levels of NGF, BDNF and GDNF in OAB patients, after lifestyle counseling (LSC) and antimuscarinic treatment (AMT) and 3) to correlate urinary levels of NFs with the severity of symptoms. METHODS Urine samples from 25 female OAB patients were collected at baseline, 3 months (after LSC) and 6 months (after 3-month AMT: oxybutynin chloride ER, 10 mg/d). Urine samples from 20 healthy women were also collected. Samples were processed for ELISA analysis of NGF, BDNF and GDNF. Urinary content of NFs was normalized against creatinine concentration. All patients completed a 7-day bladder diary combined with modified Indevus Urgency Severity Scale at baseline, 3 months and 6 months. Bladder condition was assessed using the number of urgency episodes per week (NUE/w). RESULTS At baseline, urinary NGF and BDNF were significantly higher in patients, compared to controls (NGF: 485±493 vs 188±290, p=0.006; BDNF: 792±641 vs 110±160, p<0.001). In contrast, urinary GDNF showed no statistical significant differences between both groups (790±788 vs 1220±514). After LSC, there was a decrease in urinary NGF (485±493 to 320±332) and BDNF (792±641 to 432±589, p=0.013). After AMT, NGF decreased to 180±238, while BDNF had a further reduction to 147±265. At 6 months, NGF and BDNF were significantly lower than at baseline (p<0.05). On the contrary, there was no significant variation in GDNF. At baseline, the mean NUE/w was 68±9. After LSC, there was a decrease to 56±9 (p<0.05), and, after AMT, there was a further reduction to 35±14 (p<0.05). A significant correlation was only found between BDNF and NUE/w variations, from baseline to 6 months (r=0.607, p<0.01). Using ROC analysis, the area under the curve was higher for BDNF (0.88) compared to NGF (0.75). CONCLUSIONS In OAB patients, urinary NGF and BDNF levels were significantly higher, compared to controls, while no significant differences were found for GDNF. Urinary NGF and BDNF significantly decreased after AMT. This variation was more pronounced for BDNF. These data suggest NFs as key elements in the pathogenesis of OAB. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e794 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tiago Antunes-Lopes Porto, Portugal More articles by this author Rui Pinto Porto, Portugal More articles by this author Sérgio Carvalho-Barros Porto, Portugal More articles by this author Francisco Botelho Porto, Portugal More articles by this author Paulo Diniz Porto, Portugal More articles by this author Carlos Martins-Silva Porto, Portugal More articles by this author Célia Duarte-Cruz Porto, Portugal More articles by this author Francisco Cruz Porto, Portugal More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...