1952 URINARY NEUROTROPHINS – POTENTIAL BIOMARKERS OF OVERACTIVE BLADDER

Abstract

You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Non-Neurogenic Voiding Dysfunction1 Apr 20111952 URINARY NEUROTROPHINS – POTENTIAL BIOMARKERS OF OVERACTIVE BLADDER Tiago Antunes-Lopes, Rui Pinto, Sérgio Carvalho-Barros, Paulo Dinis, Carlos Martins-Silva, Célia Duarte-Cruz, and Francisco Cruz Tiago Antunes-LopesTiago Antunes-Lopes Porto, Portugal More articles by this author , Rui PintoRui Pinto Porto, Portugal More articles by this author , Sérgio Carvalho-BarrosSérgio Carvalho-Barros Porto, Portugal More articles by this author , Paulo DinisPaulo Dinis Porto, Portugal More articles by this author , Carlos Martins-SilvaCarlos Martins-Silva Porto, Portugal More articles by this author , Célia Duarte-CruzCélia Duarte-Cruz Porto, Portugal More articles by this author , and Francisco CruzFrancisco Cruz Porto, Portugal More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.2156AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Nerve Growth Factor (NGF) may constitute a useful biomarker of OAB syndrome. Less is known about the role of Brain Derived Neurotrophic Factor (BDNF) in lower urinary tract function, although this is also an ubiquitous neurotrophin. Here, we described urinary NGF and BDNF in healthy volunteers to investigate physiological pattern of secretion and gender differences. Additionally, we assessed the urinary levels of NGF and BDNF in OAB patients, before and after life-style counselling, and correlated them with the severity of symptoms. METHODS Urine samples from 40 healthy volunteers (20 men and 20 women) were collected in the morning, afternoon and evening. The procedure was repeated 3 months later. Urine samples from 17 naive OAB women were collected at baseline and 3 months after life-style counselling. NGF and BDNF content was determined by ELISA and normalized against creatinine (Cr) concentration. For each OAB patient the severity of symptoms was accessed using Indevus Urgency Severity Scale (IUSS). RESULTS In healthy volunteers, urinary NGF and BDNF levels were constantly low, despite the time of urine collection (morning, afternoon and night). No differences were found between genders. BDNF/Cr ratio was significantly higher at baseline in OAB patients compared to healthy volunteers (980±1775 vs 110±160, p<0.01). NGF/Cr was also higher in OAB patients (423±526 vs 188±290, p=0.072). Wet OAB patients had higher urinary BDNF levels (1795±2874 vs 536±542). No such finding was found with NGF. Life-style counselling decreased urinary BDNF/Cr (980± 1775 vs 399±488, p=0.078) and NGF/Cr (423±526 vs 231±275, p=0.179). However, at this time point, BDNF/Cr levels were still significantly higher than in healthy volunteers. A significant correlation was only found between BDNF/Cr ratio and IUSS score variations (r=0.684, p<0.01). CONCLUSIONS As far as we know, this is the first comprehensive study of urinary NGF and BDNF in healthy volunteers. In this group, NGF and BDNF levels had no circadian rhythm and were persistently low, irrespective of gender. In OAB patients, urinary BDNF/Cr and NGF/Cr were high and decreased after a 3-month non-pharmacological intervention. Only BDNF/Cr had a significant correlation with IUSS score variation. The differences observed between OAB patients and controls clearly point that urinary neutrophins can be potential biomarkers of OAB syndrome. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e780-e781 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Tiago Antunes-Lopes Porto, Portugal More articles by this author Rui Pinto Porto, Portugal More articles by this author Sérgio Carvalho-Barros Porto, Portugal More articles by this author Paulo Dinis Porto, Portugal More articles by this author Carlos Martins-Silva Porto, Portugal More articles by this author Célia Duarte-Cruz Porto, Portugal More articles by this author Francisco Cruz Porto, Portugal More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...