Abstract
Objectives Preeclampsia (PE) is a specific disorder of pregnancy associated with hyperuricemia, higher levels of pro-inflammatory cytokines, enhanced leukocyte activation and oxidative stress. Adenosine deaminase (ADA) is an enzyme present in cells of the immune system, and is considered an indicator of cellular inflammation. This study aimed to compare serum levels of ADA with TNF-alpha and IL-1beta produced by peripheral blood mononuclear cells (PBMC) in pregnant women with PE, in normotensive pregnant (NT) and in non-pregnant (NP) women. Methods Sixty women with PE and 30 NT paired by gestational age were evaluated. Twenty healthy NP women matched for age with pregnant women were included as a control group. PBMC were obtained from the three groups studied and cultured with or without lipopolysaccharide (LPS) for 18h at 37 °C, and TNF-alpha and IL-1beta production was assessed in the supernatant of cultures by enzyme-linked immunosorbent assay. ADA and uric acid serum concentrations were determined by colorimetric methods. Differences between groups were analyzed by non-parametric tests with a significance level of 5%. Results Serum levels of ADA were significantly higher in PE group compared with NT and NP groups. A positive correlation between ADA and uric acid levels was detected in preeclamptic women ( r = 0.4082; p = 0.0024). There was no significant difference in relation to ADA levels when PE patients were classified in early and late-onset PE. The endogenous production of IL-1beta and TNF-alpha by PBMC was significantly higher in PE group than in NT and NP women, showing the activation state of these cells in PE. Conclusions The increased levels of ADA associated with hyperuricemia and proinflammatory cytokines suggest that this enzyme plays an important role in the systemic inflammation observed in PE. Disclosures V.S. Giorgi: None. V.T. Borges: None. S.S. Witkin: None. C.F. Bannwart-Castro: None. J.C. Peracoli: None. M.T. Peracoli: None.
Published Version
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