Abstract
Enzyme assay methods are widely used in disease diagnostics. These methods, which are carried out in 96-well microplates, are also routinely taught in laboratory courses. This chapter, however, describes an enzyme-linked immune-sorbent assay (ELISA) experiment on a microfluidic device. The ELISA experiment described here is a kinetic assay format, which has advantages over the end-point version. The chapter begins with the importance of microfluidic kinetic ELISA. The following sections cover topics related to the design of a paper microfluidic device, the chemicals and materials required, and the hazards associated with completing this experiment. The experimental section includes a step-by-step procedure for surface modification of a glass channel, the ELISA procedure, as well as data collection and analysis. Additional notes that may help instructors and students are also highlighted. Assessment questions are included to help judge students’ knowledge of the experiment. References at the end of the chapter may be helpful for further reading.
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