Abstract
This chapter presents a study to determine the expression of Y7 idiotope on IgM molecules obtained from human cord sera. Human IgM DJ was used in this study as a standard for the Y7 idiotope as well as total IgM quantification. It was isolated from the serum of a patient with Waldenstrom macroglobulinemia by means of euglobulin precipitation, followed by gel-exclusion chromatography on a Superose-6 column. The results defined Y7 idiotope as a natural cross-reactive idiotypes (CRI), by showing a relatively high incidence of Y7 idiotope expression on IgM isotype in all cord sera tested. The Y7 idiotope was substantially expressed (2–13%) on cord sera IgM, in all individual sera tested. The results provide additional experimental support for the high frequency of CRI expression within neonatal immunoglobulins, which may represent a structural basis for high mutual idiotypic connectivity of newborn IgM.
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