Abstract
Because cultured fibroblasts from patients with cystic fibrosis (CF) and their obligate carriers (CR) have been shown to have increased oxygen uptake, we reasoned that purified peripheral blood monocytes from CF and CR might show increased radical formation compared to age and sex-matched controls (CT). Monocytes were purified, counted and placed in media containing 10-6 M luminol. Chemiluminescence after stimulation 1) by opsonized zymosan particles or 2) by adherence to glass liquid scintilation vials was measured by a liquid scintilation counter and expressed as peak CPM/monocyte. When maximally stimulated with pre-opsonized zymosan particles monocytes from CF, CR and CT generated essentially equivalent amounts of chemiluminescence. However, after stimulation by adherence to glass scintillation vials, monocytes from both CF (n = 5) and CR (n = 5) generated significantly greater amounts of chemiluminescence than their respective controls (CF: 3.5 ± 0.5 vs CT: 1.2 ± 0.6, p = .001; CR: 2.7 ± 0.8 vs CT: 1.3 ± 0.5, p = .01). Moreover, peak chemiluminescence produced by CF monocytes after adherence was significantly higher than CR monocytes (p < .05). Thus the monocytes from both obligate carriers and patients with CF respond to membrane stimulation triggered by attachment to glass with increased oxygen radical formation as measured by chemiluminescence. This assay may be useful for detecting the carrier state of cystic fibrosis.
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