Abstract
The cytotoxic effect of activated lymphocytes (effector cells) on other cells (target cells) in vitro is believed to offer a model for lymphocyte-mediated tissue damage as occurring in vivo in delayed hypersensitivity, allograft, or tumor rejection and certain autoimmune estates. Measurement of release of 51Cr from labeled target cells exposed to lymphocytes provides a convenient assay system. This chapter describes this assay for the combination 51C r-labeled chicken erythrocytes-human lymphocytes, the latter activated either by phytohemagglutinin or by rabbit antibody to chicken erythrocyte antigen. Most of the rules which determine the course of these cytotoxic reactions have also been found to hold for other models in which 51Cr-labeled tissue culture cells have been used as target cells.
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