16SrRNA sequencing as tool for identification of Salmonella spp isolated from human diarrhea cases

Abstract

This study was conducted in different location of Babylon, (75) clinical specimens were collected, obtained from patients suffering from severe diarrhea were attended to Merjan teaching hospital and Private clinics in Babylon province, and suffer from severe diarrhea that was associated with fever as diagnosed by physician, The period of specimens collection extended from September 2017 to January 2018. Results showed that out of 75 studied specimens of patients, 14 specimens were Salmonella positive (18.66 %). Salmonella isolates were isolated and identified by using bacterial culturing on selective media and confirming tests such as 16S rRNA gene amplification, all results of these diagnosis methods referred to all isolates belong to Salmonella spp. Depending on sequencing method for nucleotide sequence for 660 bp of 16S rRNA gene we sent only 10 isolates to registration these sequences in Gene Bank-NCBI for diagnosis of these isolates on species level and obtaining accession numbers then phylogeny. Ten accession numbers were obtained from 16SrRNA and registration of 10 sequences of 16SrRNA gene at gene bank-NCBI include one accession number (MH109326.1) for Salmonella enterica subsp. enterica serovar Typhimurium, Five accession numbers (MH156032.1, MH109512.1, MH109501.1, MH155972.1 and MH156033.1 )belong to Salmonella enterica sub.sp enterica serovar Typhi. While Salmonella enterica sub.sp enterica serovar Paratyphi recorded under two accession number (MH155974.1 and MH156039) and Salmonella enterica sub.sp enterica serovar Enteritidis recorded under two accession number (MH109386.1 and MH155973.1) respectively.