16 s rRNA sequencing for Pseudomonas aeruginosa isolated form clinical samples

Abstract

Pseudomonas aeruginosa is a species of bacteria that can be found in soil and water. It can cause nosocomial infections as well as infections in blood, lungs, and other body organs. The “S” in 16S is a coefficient of sedimentation, or an index indicating a macromolecule's downward velocity in a centrifugal field, and It's part of the 30S subunit of the prokaryotic ribosome. Primers are designed after the analysis is completed. The extracted DNA is preserved and quantitatively tested for purity and quantity. The PCR mixture is made and used in the PCR machine. The DNA fragments are then separated using gel electrophoresis. Mutational analysis is performed using PCR product sequencing. The P. aeruginosa strain shares and differs from other strains in the same genus. The article's goal is to look at the P. aeruginosa strain.