Abstract
Head and neck squamous cell carcinoma (HNSCC) is known as one of the six most common human cancers mainly caused by consumption of tobacco and alcohol. There is also a genetic factor; however, the genetic markers are not yet established. Our objectives were to: Validate the genetic signature of molecular targets expressed by tumors in HNSCC. Determine potential biomarkers for earlier detection, potential therapies and prediction of patients’ survival. The HNSCC patients were recruited to the study in the Greater Poland Cancer Centre in 2010. Oral cancer and normal epithelium tissue taken at a minimum of 2 cm distal from the tumors’ margins from 41 patients were used for analysis by Cancer Pathway Finder array and followed with real-rime PCR. Analysis indicated up-regulation of 11 genes including KRT14, ACLY, MCM2, SKP2, STMN1, CDC20, SNAI2, MKI67, SLC2A1, BCL2L11, IGFBP3 (P < 0.05) suggesting altered regulation of cell cycle, cell senescence, metabolism, apoptosis and hypoxia. Five years patient follow up survival analysis indicated that SKP2, KRT14, FOXC2, Acly, PGF, OCLN, CDH2, LDHA, VEGFC, BCL2L11, CA9 genes expression was significantly associated with survival of the patients. Our data indicate that there is significant activation of several cellular pathways in tumor tissue that should be further investigated. Importantly, observed significant association between the expression of SKP2, KRT14, FOXC2, Acly, PGF, OCLN, CDH2, LDHA, VEGFC, BCL2L11, CA9 and survival indicate that the level of the expression of these genes in tumor tissue may predict the survival of the patient.
Published Version
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