Abstract
The application of compact disc (CD)-type sensor devices to biological sensing has been investigated. On these devices, solutions are provided, including the transport of samples into microchannels by centrifugal force without using any pump and where microanalyses can be conducted. These powerful aspects of such devices have enabled them to be used for simple and high-throughput analyses. We have developed CD-shaped microfluidic devices that can be used as a biosensor for various applications. Three applications are reported here: to single cell isolation and detection of a cell-specific gene, discrimination of an entrapped cell by staining, and high-throughput analyses based on the enzyme linked immunosorbent assay (ELISA). In this chapter, we summarize the applications of biosensors performed on CD-shaped microfluidic devices such as gene analysis of isolated cells based on a specialized polymerase chain reaction, cell staining for nonadherent cells, and rapid ELISA.
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