Abstract

This chapter begins with a description of the atomic mass unit, or dalton, defined as 1/12 the mass of a carbon-12 atom. It then proceeds to define molecular weight, in daltons, and the gram molecular weight. It then discusses the mole in chemistry and Avogadro’s number. This chapter then defines concentration in terms of molarity and how to make up solutions and dilutions of solutions to achieve desired concentrations. It describes the Fick dilution principle for determining unknown volumes. This chapter then considers kinetics in terms of forward and reverse rate constants, defining the equilibrium constant as the ratio of forward to reverse rate constants. It discusses kinetics in terms of potential energy along the reaction coordinate, the distance along the minimum free-energy path from reactants to products. This chapter then derives the Michaelis–Menten formula for enzyme kinetics and discusses how enzymes, in general, catalyze reactions. It ends with a derivation of the double-reciprocal plot for enzyme kinetics.

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