Abstract
To compare the results of low resolution typing by sequence specific primers and sequence specific Oligonucleotide probes on Luminex platform. Prior to switching over to Luminex we performed initially low resolution HLA typing for thirty samples for ABDR antigens. We further validated HLA typing results on Luminex platform with two-third volumes of reagents. Kits from CPC Diagnostics (USA) and Innotrain (Germany) were used for SSOP and SSP typings respectively. We subsequently typed another twenty samples by both methods. Any addtional discreapancies encountered on typing over next two months shall also be presented. The results were concordant for all antigens at the A locus. One antigen was not identified at the B locus on typing by Luminex. Three typing results were discordant for DRB1 locus. There were no ambiguities in Class I typing on Luminex platform whereas six samples gave ambiguous results at B locus by SSP. We got confounding results by on Luminex platform for a sample of cord blood with maternal chimerism and also for DR typing of three samples. SSOP typing gave fewer ambiguities for class I typing. For class II typing specially in cases of cord blood with maternal contamination we prefer SSP which gave clear results.
Published Version
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