Abstract
You have accessJournal of UrologyProstate Cancer: Basic Research VIII1 Apr 20101442 PROFILING TUMOUR INFILTRATING LYMPHOCYTES AND THEIR ACTIVATION STATUS IN PROSTATE CANCER Nieroshan Rajarubendrah, S. Deb, G. Whitty, Pavel Sluka, Damien Bolton, and Ian Davis Nieroshan RajarubendrahNieroshan Rajarubendrah Heidelberg, Melbourne, Australia More articles by this author , S. DebS. Deb Heidelberg, Melbourne, Australia More articles by this author , G. WhittyG. Whitty Heidelberg, Melbourne, Australia More articles by this author , Pavel SlukaPavel Sluka Heidelberg, Melbourne, Australia More articles by this author , Damien BoltonDamien Bolton Melbourne, Australia More articles by this author , and Ian DavisIan Davis Heidelberg, Melbourne, Australia More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1135AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The immune system has been reported as playing an important role in the pathogenesis of prostate Cancer (PCa) using mechanisms of local immunosuppression. Regulatory T cells (Tregs), a subpopulation of T-lymphocytes, independently regulate immune responses and are crucial in controlling immune homeostasis. We examined T cell subset proportions and their activation and maturation status in the blood and tissue of patients with PCa, with a view to ascertaining differences related to the presence or absence of prostate cancer, using BPH tissue as a control. METHODS Fresh tissue from patients with PCa and benign prostatic hyperplasia (BPH) was obtained with matching blood. Lymphocytes in the blood were isolated using Ficoll separation and tissue lymphocytes were studied as either single cell suspensions or in tissue frozen sections. Single cell suspensions of the tissue and matching blood lymphocytes stained for CD3, CD4, CD8, CD45RA, CD45RO, CCR7, CD69, CD107a and FoxP3 were examined using flow cytometry, whilst frozen sections were stained for CD4, CD8 and FoxP3 using immunohistochemistry (IHC). RESULTS Tregs in PCa tissue were present at variable levels compared to those in blood. Some patients had very few tumour-infiltrating lymphocytes (TIL) and no correlations could be drawn about relative Treg proportions. When TIL were prominent, Treg proportions were up to three times higher than in corresponding peripheral blood, implying a possible role in local immunosuppression. There were no correlations observed between Treg proportions and conventional clinical prognostic markers. In contrast Treg proportions in BPH tissue were similar to those in blood. TIL in PCa showed a decrease in naïve and central memory cells compared to blood, while terminal effector T cells and effector memory T cells were increased. CD8 T cell activation was upregulated. CONCLUSIONS TILs are often absent in PCa. When present, Treg proportions were higher than blood and also had increased levels of terminal effectors and effector memory cells. In contrast Treg proportions in BPH tissue were similar to those in blood. These findings may have implications for local tissue immune regulation and immunotherapy. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e555-e556 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Nieroshan Rajarubendrah Heidelberg, Melbourne, Australia More articles by this author S. Deb Heidelberg, Melbourne, Australia More articles by this author G. Whitty Heidelberg, Melbourne, Australia More articles by this author Pavel Sluka Heidelberg, Melbourne, Australia More articles by this author Damien Bolton Melbourne, Australia More articles by this author Ian Davis Heidelberg, Melbourne, Australia More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
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