1408Evaluation of Legionella Diagnostic Testing by Urinary Antigen, Culture, and PCR

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The clinical database was searched for Legionella urinary antigen (UAG), culture (CUL), and polymerase chain reaction (PCR) ordered Mar 2010 to Dec 2013 at Cleveland Clinic. Each test could be ordered individually, or CUL and PCR could be ordered as a single set (CUL+PCR). UAG was performed by the Legionella Urinary Antigen EIA (Binax) and the laboratory developed PCR was run on the LightCycler (Roche) using Legionella pneumophila primers and probes. For cases where all 3 test methods were performed and at least one was positive, the medical record was reviewed for relevant clinical and epidemiologic factors.