Abstract
Publisher Summary A variety of strategies have been used to express recombinant structural and functional RNAs in mammalian cells, including the use of classical mRNA promoters and promoters from such structural/functional RNAs as rRNAs, tRNAs, and snRNAs. Many factors contribute to achieving optimal activity of recombinant structural/functional RNAs, including the amount of transcript produced in the target cell type, the stability and intracellular localization of the recombinant transcript, and preservation of the biological activity of the effector RNA in the context of other sequences contained in the recombinant transcript. This chapter reviews the different promoters used to express effector RNAs in mammalian cells. The chapter discusses the intracellular localization of the different transcription units and describes strategies to improve the stability and to preserve the activity of the different types of effector RNAs in the context of recombinant transcripts.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call