Abstract
This chapter explores that a key to the understanding of the immune system is the ability to separate and purify lymphoid cells so as to study their characteristics, functions, and interactions. It discusses that a major advance in achieving this aim has been the development of techniques which take advantage of the discriminatory power of antibodies to select for cells bearing a particular type of surface antigen. In many cases this results in the separation of functional lymphocyte subsets since these are usually characterized by particular surface antigen markers. For example, anti-IgM antibodies can be used to separate B cells, most of which carry surface immunoglobulin molecules, from T cells, which do not carry surface immunoglobulin molecules. This chapter describes the use of immunoadsorbent techniques to separate lymphoid cells. In these methods antibodies specific for a lymphocyte surface component are bonded to solid phase matrices to provide a selective anchor for isolating given subpopulations.
Published Version
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