Abstract
Publisher Summary This chapter discusses the identifiability of two-state excited-state processes with known concentration dependence in the forward process. It is demonstrated that the decay curves in a fluorescence decay surface do not need to be normalized to perform a global analysis in terms of the parameters of interest. The normalization of decay curves is a tedious and sometimes even impossible task. The steady-state spectra can be used afterward to obtain the species-associated spectra. The chapter describes the excited-state processes with time-independent rate constants. The identifiability equations and the identifiability of a two-state excited-state process are discussed in the chapter.. The chapter discusses the way the species-associated spectra can be calculated using the information obtained from the analysis of the decay surface and steady-state spectra. The chapter presents some indications to extend existing global analysis programs to compartmental analysis. The determination of the ground-state equilibrium constant is then discussed in the chapter.
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