Abstract
The fluorescence decay analysis of intramolecular two-state excited-state processes with added quencher is discussed in terms of compartments. The kinetics specifying the two excited-state species concentrations are derived. The fluorescence decay surface is expressed in terms of the system parameters, namely the rate constants and the spectroscopic parameters b 1 and c 1 . b 1 and c 1 are respectively the relative absorbance and the normalized spectral emission weighting factor of species 1. The report investigates the prerequisites for obtaining the unique set of system parameters
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