Abstract
ABSTRACT 1,3-Dioleoyl-2-palmitoylglycerol (OPO) is a kind of structured triglyceride which could instead human milk fat (HMF) for the fat of infant formula, this kind of structured triglyceride is good at absorbing easily. The aim of this paper was to produce OPO though three different processing technologies. The raw material for the first method (Method 1) and second method (Method 2) was palm oil, and their catalyst is Novozym 435 and Lipozyme TL IM. The difference was the Method 1, which obtained tripalmitin (PPP) by acidolysis palm oil and palmitic acid (PA), then obtained OPO by acidolysis PPP and oleic acid (OA), Method 2 was acidolysis palm oil and mixed fatty acids directly without crystallization. The third method (Method 3) produced palm stearin (PS) enriched in PA by catalyzing palm stearin with sodium methoxide firstly, and then palm stearin enriched in PA and mixed vegetable oils catalyzed by Lipozyme TL IM. The content of OPO and sn-2 position PA of three methods was higher than 40.34% and 39.34%. The relative content of PA located at the sn-2 position and OA located at the sn-1, 3 position was higher than 59.97% and 66.01%. Among the three methods, the methods related to enzyme appeared safer, and Method 3 was easier for manufacturing OPO and saved time, so it should be more suitable for industrial production.
Highlights
Human milk is the ideal source of nutrients for newborn infants, which could supply energy and essential nutrients to infants.[1]
6.40 ± 0.08 68.12 ± 0.13 6.03 ± 0.07 64.70 ± 0.09 7.73 ± 0.09 73.79 ± 0.10 a The OPO was synthesized by palm oil with palmitic acid (PA) and oleic acid (OA). b The OPO was synthesized by palm oil and mixed fatty acids. c The OPO was synthesized by palm stearin (PS) and mixed vegetable oils. d The yield of OPO which separate from lipase. e The yield of OPO after purification. f The amount of purified OPO account for the unpurified OPO
We supposed that a reasonable proportion of fatty acids in the acyl donor has a positive effect on the OPO content
Summary
Human milk is the ideal source of nutrients for newborn infants, which could supply energy and essential nutrients to infants.[1]. We have to add structured triglycerides in infant formula as energy supplement. It is about 3–5% HMF in human milk and more than 98% HMF in it is triacylglycerols (TAGs).[3,4] The major saturated fatty acid in human milk fat is PA, which accounts about 20–25% of the total fatty acids,[5] and approximately 70% of PA is located at the sn-2 position of TAGs. It is about 3–5% HMF in human milk and more than 98% HMF in it is triacylglycerols (TAGs).[3,4] The major saturated fatty acid in human milk fat is PA, which accounts about 20–25% of the total fatty acids,[5] and approximately 70% of PA is located at the sn-2 position of TAGs Other fatty acids such as OA and linoleic acid, which are unsaturated fatty acids, are mainly located at the sn-1, 3 positions.[6] a great number of TAGs in HMF are the form of ABA (A is unsaturated fatty acid and B is saturated fatty acid). The distribution of unique fatty acids in these TAGs has a great influence on the improvement of infants’ growth
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