Abstract
You have accessJournal of UrologyProstate Cancer: Basic Research (VI)1 Apr 20131324 PIRIN DOWN-REGULATES THE EAF2/U19 SIGNALING AND RETARDS THE GROWTH INHIBITION INDUCED BY EAF2/U19 IN PROSTATE CANCER CELLS Zhongjie Qiao, Dan Wang, and Zhou Wang Zhongjie QiaoZhongjie Qiao Pittsburgh, PA More articles by this author , Dan WangDan Wang Pittsburgh, PA More articles by this author , and Zhou WangZhou Wang Pittsburgh, PA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.2678AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES EAF2/U19, as the tumor suppressor, has been reported to induce apoptosis of LNCaP cells and suppresse AT6.1 xenograft prostate tumor growth in vivo, and its expression level is down-regulated in advanced human prostate cancer. EAF2/U19 is also a putative transcription factor with a transactivation domain and capability of sequence-specific DNA binding. Identification and characterization of the binding partners and regulators of EAF2/U19 is essential to understand its function in regulating apoptosis/survival of prostate cancer cells. METHODS Yeast two-hybrid assay was performed to identify the novel binding partners of EAF2/U19. Immunoprecipitation was performed to confirm the interaction between EAF2/Pirin. Colony formation assay was used to examine the cell growth. RESULTS Through the yeast two-hybird screening system, we identified Pirin, a highly conserved nuclear protein which originally isolated as an interactor of NF1/CTF1 transcription factor, as the binding partner of EAF2/U19. We further confirmed the interaction between EAF2/Pirin by immunoprecipitation in mammalian cells. Although overexpression of Pirin did not change the localization and expression pattern of EAF2/U19, the overexpressed Pirin decreased the expression level of EAF2/U19 in prostate cancer cell lines including LNCaP and PC3 and 293T. Furthermore, Colony formation assay was performed to confirm that overexpression of EAF2/U19 suppressed the colony formation, and co-expression of Pirin with EAF2/U19 significantly retarded the growth inhibition induced by overexpression of EAF2/U19. CONCLUSIONS Our study discovered a new binding partner and regulator of EAF2/U19, which leads to the further understanding of the EAF2/U19 signaling pathway in regulating apoptosis and proliferation of prostate cancer cells, and potentially contributes to discover the novel therapeutic treatments to inhibit tumor growth. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e541 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Zhongjie Qiao Pittsburgh, PA More articles by this author Dan Wang Pittsburgh, PA More articles by this author Zhou Wang Pittsburgh, PA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
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