13. The Amount of Melanomacrophage centres (MMC) in Liver and Kidneys of Tilapia (Oreochromis niloticus) Maintained in Various Population Density

Nurul Hadi,Dwina Aliza,Razali Daud

doi:10.21157/j.med.vet..v11i2.4379

Abstract

The aim of this research was to find out the amount of melanomacrophage centres (MMC) in liver and kidney of nile tilapia (Oreochromis niloticus) fish reared in high dense population. Fifty nile tilapia were grouped in four treatment groups, group I contain 5 fish, group II contain 10 fish, group III contain 15 fish and group IV contain 20 fish. Treatment was carried out for 24 hours. Fish was necropsied to collect the liver and kidney, fixed in NBF 10% then proceed to histopathological method using hematoxilin and eosin for staining. Histopathological observation was done microscopically, and MMC was captured by photomicrograph. The data were analyzed descriptively. The result showed that the average of MMC number in fish liver in group K1, K2, K3, and K4 were 9.6, 11.3, 12.6, and 13.2. While the average of MMC number in fish kidney were 5.5, 7.7, 8.5, and 9.2, respectively. The higher the dense of population the higher the amount of MMC found in liver and kidney of nile tilapia.

Highlights

Ikan nila merupakan salah satu dari ikan air tawar yang paling banyak dibudidayakan
The aim of this research was to find out the amount of melanomacrophage centres (MMC) in liver and kidney of nile tilapia (Oreochromis niloticus) fish reared in high dense population
Fifty nile tilapia were grouped in four treatment groups, group I contain 5 fish, group II contain 10 fish, group III contain 15 fish and group IV contain 20 fish

Summary

MATERI DAN METODE PENELITIAN

Sebanyak 50 ekor ikan nila yang dibagi atas 4 kelompok perlakuan. Kelompok K1 adalah kelompok kontrol terdiri atas 5 ekor ikan, kelompok K2 terdiri atas 10 ekor ikan, kelompok K3 terdiri atas 15 ekor ikan, dan kelompok K4 terdiri atas 20 ekor ikan. Akuarium diisi air dengan ketinggian 30 cm. Sistem pemeliharaan menggunakan sistem air statis dan selama pemeliharaan ikan diberikan pelet sebanyak 3 kali sehari. Setelah dilakukan perlakuan selama 24 jam kemudian dilakukan pengambilan sampel organ hati dan ginjal. Ikan diambil sebanyak 3 ekor dari masing-masing perlakuan dalam wadah pemeliharaan lalu dibius menggunakan klorofom.

Pembedahan untuk pengambilan sampel organ hati dan ginjal dilakukan dengan

HASIL DAN PEMBAHASAN

DAFTAR PUSTAKA

Fakultas Perikanan dan Ilmu Kelautan

Populasi Terhadap Gambaran Patologi