129 Effect of different nanoparticles on the SF21 cell membrane transport properties to water and glycerol

Abstract

Many researchers focus on the cell toxicity of nanoparticles and drug transportation with the nanoparticles. Recently cryobiologists found that the efficiency of cryopreservation of many cells and tissues is good when nanoparticles added to the cryoprotectant solution. However, the mechanism is not understood. Previous study demonstrated that the ice growth is accelerated and devitrification temperatures of cryoprotectant solutions are significantly lowered in these nanoparticles cryoprotectant solutions, and the nanoparticles could change the structure and property of cell membrane. To obtain the optimal protocols for freezing, thawing, and addition/removal of cryoprotectant agent (CPA), the membrane transport properties need to be determined. In our study, we applied the microperfusion system to the measurement of the SF21 cell membrane transport properties to water and glycerol with hydroxyapatite TiO 2 SiO 2 and AlO 3 at 20 °C. We found that the SF21 cell membrane transport properties to water and glycerol is significantly different with nanoparticles. The finding can be used to improve the efficiency of SF21 cell conventional and vitrified cryopreservation, also we should pay attention to the application of the nanoparticles in cryogenics. Source of funding: National Natural Science Foundation of China (No. 51076149). Conflict of interest: None declared. jywong@mail.ustc.edu.cn