Abstract
Fetal rabbits at 29 days gestation were delivered both from does made hyperglycemic with IV streptozotocin (S) (75mg/kg) prior to conception ε from control (C) does. Fasting maternal serum glucose levels at 29 days gestation were 153±43.7mg/dl in S does ε 99.4±19.7mg/dl in C. Fetal glucose levels immediately after cesarean section were 84.8±10.3 S ε 75.3±23.1 C. Lungs from both groups of fetuses were homogenized, an aliquot reserved for parenchymal phospholipid determinations, ε microsomal fractions isolated from the remainder by density gradient centrifunation. Microsomal synthesis of phosphatidylcholine (PC) using 14C choline chloride, phosphatidylinositol (PI) using 3H myoinositol, ε phosphatidylglycerol (PGL) from 14C αglycerol-3-phosphate as substrates were determined in vitro ε expressed as pmole/mg protein/min. Fetal lung microsomal PC synthesis during maternal hyperglycemia was unchanged from control; however, synthesis of minor phospholipids showed a decrease in P1 ε an increase in PGL synthesis during maternal hyperglycemia. Our results differ from reports of decreased PC in increased glucose environment in lung tissue culture & from reports of decreased PGL synthesis in adult lung tissue from streptozotocin treated rabbits. (Supported by HD-05292 and HD-04380)
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