Abstract
Aim To investigate changing protocol for HLA typings on related Bone Marrow (BM) Donors. Current protocol: Recipient typed at low res with ABDRDQ kit. Siblings typed at low res with ABDR kit. Matched Donor at low prompts BM program to select donor for further testing which initially includes high res DR for Recipient & Donor. If matched, low res C & high res A, B & C performed for matching pair. Methods DNA extraction using manual salting-out extraction kit by Qiagen. PCR-SSP using low & high resolution primer kits by One Lambda & Olerup. Results Typing performed on Recip: A ∗ 01,29,B ∗ 57,56,DRB1 ∗ 07,01,B4 ∗ ,DQB1 ∗ 02,05. Typings on 3 sibs all resulted in matches at low res ABDR. Donor selection made by BM program, we proceeded with high DR typing to confirm match. Confirmed DRB1 ∗ 07:01, 01:02, followed by low C typing: results C ∗ 06,16. Performed high res A, B & C for Recipient & Donor. Questioned likelihood of 4 sibs matching at all loci. Unlikely frequency, prompted (outside protocol) high DR on other 2 siblings. Two sibs matched at high DRB1. For education, we confirmed original typings & then low C & DQ to see if all sibs matched. We discovered 1 sib had antigen MM at DQ. Conclusions Overall, we concluded that the ultimate benefit would have been discovering DQ MM initially that would otherwise have been missed. Now, reconsidering approach to related BM Donors.
Published Version
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