Abstract
This chapter summarizes approaches and provides representative examples for the selective visualization of specific cell surface saccharides. Over the years, numerous experimental approaches have been instituted for the ultrastructural localization of cell surface polysaccharide coats. For the sake of simplicity, these approaches are divided into three major categories: (1) direct localization, whereby the marker used either possesses innate affinity toward a given surface saccharide or has been chemically altered with an appropriate sugar-specific binder, (2) indirect localization, whereby the interaction of a given marker with a cell surface saccharide depends upon the noncovalent mediation or bridging between the surface site and electron dense marker, and (3) component modified localization whereby the cell surface saccharide is altered by chemical or enzymic means and the modified saccharide can then be specifically localized either directly or indirectly.
Published Version
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