11-cis Retinol as a Substrate for Cone Dark Adaptation

Abstract

We have determined the effectiveness of 11-cis retinol as a substrate for visual pigment formation in intact vertebrate cone and rod photoreceptors and measured opsin-mediated transducin activation by 11-cis retinol. Methods were of two types. Firstly, visual pigment absorbance spectra were measured microspectrophotometrically in single cone and rod photoreceptor outer segments before and after bleaching of the native visual pigment and following subsequent treatment with 11-cis retinal and 11-cis retinol. Secondly, we expressed human and salamander cone and rod opsins in COS cells and then tested in a cell free assay the effects of these retinoids on the activation of transducin by opsin. We show that 11-cis retinol promotes pigment formation in bleached red and blue salamander cones but not in bleached salamander red or green rods. Transducin activation experiments show that 11-cis retinol acts as an inverse agonist of red and green cone opsins, but has no effect on the activity of blue cone opsins. In contrast, 11-cis retinol acts as an agonist of rod opsin. We conclude that cones have a mechanism for handling retinoids and regenerating visual pigment that is different from rods. 11-cis Retinal and 11-cis retinol are usable substrates for cone pigment regeneration and dark adaptation as both retinoids promote pigment regeneration and neither elicits activation of the transduction cascade by opsin. On the other hand, 11-cis retinol is not useful for rod function since it does not promote pigment regeneration and its opsin-mediated activation of rod transducin may slow the rate of rod dark adaptation.