Abstract

You have accessJournal of UrologyKidney Cancer: Localized I1 Apr 20121109 MEASURING THE PROTECTIVE EFFECT OF ALLOPURINOL USING ISOPROSTANE AFTER RENAL ISCHEMIA IN AN ANIMAL MODEL Christopher Keel, Zijun Wang, Janet Colli, and Benjamin R. Lee Christopher KeelChristopher Keel New Orleans, LA More articles by this author , Zijun WangZijun Wang New Orleans, LA More articles by this author , Janet ColliJanet Colli New Orleans, LA More articles by this author , and Benjamin R. LeeBenjamin R. Lee New Orleans, LA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1217AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Partial nephrectomy involves clamping the renal artery temporarily, making the kidney susceptible to ischemic damage from both warm ischemia and reperfusion injury. Isoprostane represents one potential marker of oxidative injury. The objective of this study was to determine if renal interstitial isoprostane levels can quantitate renal damage secondary to warm ischemia. A second goal is to investigate allopurinol for renoprotective abilities. We selected allopurinol as a potential nephroprotective agent because prior studies have demonstrated transplant kidneys pretreated with allopurinol suffer less damage from ischemia. METHODS After approval by the Institutional Animal Care and Use Committee, Sprague-Dawley rats (n=65) underwent insertion of a microdialysis probe into renal parenchyma to allow continuous dialysis and collection of effluent for isoprostane levels. Following clamping of the renal vessels for predefined intervals of ischemia, effluent from the BASi IV-10 Vascular Microdialysis Probe (10mm Membrane), was collected during the following time periods: preclamp (30min), clamp (15, 30, 45 and 60 min), postclamp (30min). The interstitial fluid samples were analyzed for isoprostane levels using a Cayman Chemical 8-isoprostane ELA Kit and BMG LABTECH OPTIMA microplate reader. RESULTS Clamping of the renal artery and vein produced increased isoprostane levels during the ischemic period and larger increases during reperfusion. There was a trend for increased post-clamp isoprostane levels as clamp times increased. When comparing isoprostane levels in rats which did not receive allopurinol, there were significant differences between the clamp and post clamp levels of isoprostane, when the renal hilum was clamped for 60 minutes, at the postclamp timepoint of 30 minutes (p=0.022); with allopurinol offering protection to the kidney from ischemic damage. Control group demonstrated with external trauma, isoprostane was still produced in the presence of allopurinol. CONCLUSIONS Isoprostane levels are a potential real-time marker of renal ischemia and reperfusion injury. Allopurinol administration demonstrated a trend toward renoprotective abilities in the hilar occluded kidney, in this animal model at 60 minutes. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e450 Peer Review Report Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Christopher Keel New Orleans, LA More articles by this author Zijun Wang New Orleans, LA More articles by this author Janet Colli New Orleans, LA More articles by this author Benjamin R. Lee New Orleans, LA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

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