Abstract
Functional genomics has transformed biological research in the past 2 decades. Functional genomics brings together high-throughput genetics with multiparallel analyses of gene transcripts, proteins, and metabolites to answer challenging questions posed by all completed genome-sequencing projects: i.e., what is the biological function of each and every gene? Advances in genome editing and engineering technologies and their applications, such as zinc-finger nucleases, transcription activator-like effector nucleases, clustered regularly interspaced short palindromic repeat/Cas9, and recombineering have made precise control over the genome sequence and its regulation possible across all disciplines including plant sciences. Rapid evolution of novel technologies in functional genomics has also catalyzed a new era of genomics that includes multiple approaches to functionally characterize and manipulate the regulation of genomic information. The current chapter summarizes earlier as well as novel technologies and their applications in plant functional genomics.
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