Abstract
Sequencing by hybridization (SBH) applied to dense arrays of clones achieves an unprecedented throughput of analyzed samples that can be performed inexpensively. The adaptation of this technology to an industrial setting has already produced 1 million partial cDNA sequences per month and a database of more than 4 million individual cDNA clones in the facility. This chapter discusses the present protocols and the potential application of this technology to the fields of gene discovery and gene function. SBH technology applied on an array of clones provides a progressive approach from expression screening to complete sequencing of complex libraries. The same approach is applicable for mutation detection and complete sequencing of many genes amplified by polymerase chain reaction (PCR) from thousands of individual samples.
Published Version
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