109 Influence of nebulized antibiotics on ciliary activity in vitro

Abstract

Introduction The efficacy of several inhaled drugs is well studied in the context of cystic fibrosis (CF). Their use is however extrapolated to other chronic diseases, without clear evidence of benefit. In addition, the effect of inhaled drugs on ciliary activity has rarely been studied. Previously, we validated an in vitro model for testing the effect of nebulized drugs on ciliary beat frequency (CBF) and showed negative influence of Obracin® but not of Tobi®. Objectives To examine the influence of 2 antibiotics on CBF: the approved inhalation drug Cayston® (C) (provided by Gilead) and Glazidim® (G), used off-label for inhalation in patients with specific infections, e.g. Achromobacter. Methods Non-CF nasal epithelial cells were cultured as monolayer. On day 4 to 6, confluent cell monolayers were exposed to nebulization in an aerosol chamber for 10 minutes. C was nebulized with an eFlow nebulizer plus Altera head, G with a Pari boy nebulizer plus Pari LC star head. Isotonic saline (S) was used as paired, neutral control. Images were acquired with a high-speed camera, and CBF was expressed as % of the starting value. CBF until 1 hour after nebulization was compared between the test condition and S using repeated measure mixed effect model statistics. Results Compared to S, C (n = 4) did not influence CBF (p 0.355): CBF was 96% 1 hour after nebulization of C, versus 98% for S. G (n = 3) neither influenced CBF (p 0.346): CBF was 99% 1 hour after nebulization of G, versus 109% for S. Results on days 2 and 3 were similar. Conclusion Nebulization of Cayston® and Glazidim® did not influence ciliary activitity in vitro. This may be relevant to their in vivo efficacy and safety.