103 Minimum inhibition concentration characteristic of antibiotics on Pseudomonas aeruginosa strains from patients with cystic fibrosis

Abstract

Aim Study minimum inhibitory concentrations of antibiotics on Pseudomonas aeruginosa strains from patients with cystic fibrosis (CF) in lung infection. Methods The study presents an analysis of 32 Ps. aeruginosa strains identified in sputum of 15 patients with CF. Determination of minimum inhibitory concentration (MIC) was performed with the device WalkAway-96 (Siemens, Germany). Of the 15 patients with CF, 13 have chronic pulmonary infection with Ps. aeruginosa, of whom one patient has chronic pulmonary infection with Pseudomonas two types. Results Microbiological research found that 62% of Ps. aeruginosa strains are sensitive to amikacin (MIC ≤ 16) and 81% to tobramycin (MIC ≤ 4). In 38% of cases amikacin – intermediate sensitivity (MIC = 32) and in 16% tobramycin – resistance (MIC>8). A high sensitivity (88%) showed these strains to ceftazidime (MIC ≤ 8), but in 6% cases Ps. aeruginosa was resistant to this antibiotic (MIC>16). In 53% of cases Ps. aeruginosa is sensitive to cefepime with MIC ≤ 8, other strains have intermediate sensitivity. Antibiotics from the imipenem group have high sensitivity (88%) for Ps. aeruginosa with MIC ≤ 4 and only 12% of cases show resistance (MIC>8) or intermediate sensitivity (MIC = 8). The sensitivity of Ps. aeruginosa strains to ofloxacins (ciprofloxacin MIC ≤ 1) was found in 50%. Piperacillin and piperacillin/tazobactam are antipseudomonal antibiotics with high sensitivity (MIC ≤ 16) in 81% and intermediate sensitivity (MIC = 32) in 19% of cases. Conclusion Ps. aeruginosa strains in children with CF in Republic of Moldova have a high sensitivity to antipseudomonal antibiotics, only in single cases it can be caused by multidrug-resistant patients with FC.