1029 IDENTIFICATION OF MICRORNA EXPRESSION PROFILE IN DIABETIC RATS' PENILE TISSUES AND POTENTIAL ROLE OF MIR-92A IN ERECTILE DYSFUNCTION

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research (II)1 Apr 20131029 IDENTIFICATION OF MICRORNA EXPRESSION PROFILE IN DIABETIC RATS' PENILE TISSUES AND POTENTIAL ROLE OF MIR-92A IN ERECTILE DYSFUNCTION Jun Yang, Tao Wang, Hua Xu, Shaogang Wang, Jihong Liu, and Zhangqun Ye Jun YangJun Yang Wuhan, China, People's Republic of More articles by this author , Tao WangTao Wang Wuhan, China, People's Republic of More articles by this author , Hua XuHua Xu Wuhan, China, People's Republic of More articles by this author , Shaogang WangShaogang Wang Wuhan, China, People's Republic of More articles by this author , Jihong LiuJihong Liu Wuhan, China, People's Republic of More articles by this author , and Zhangqun YeZhangqun Ye Wuhan, China, People's Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.614AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES MicroRNAs are a recently discovered class of endogenous, small, noncoding RNAs, which play regulatory roles in several biological processes. However, the role of miRNAs in erectile dysfunction (ED) is currently completely unknown. This study was designed to identify specific miRNA whose expression is altered in diabetes-related ED and explore the role of miR-92a in diabetes-induced ED. METHODS A streptozotocin-induced diabetes rat model was established and the erectile function was evaluated by electrical stimulation of cavernous nerve twelve weeks after diabetes induction. Global miRNAs expression in penile tissues from normal rats and diabetic ED rats were measured using Agilent micoRNA microarray and the aberrantly expressed miRNAs were further confirmed by quantitative real-time polymerase chain reaction (PCR). The mRNA and protein expression level of endothelial nitric oxide synthase (eNOS) were detected by quantitative real-time PCR and western blot. MiR-92a functional assays were performed on rat aortic endothelial cells. RESULTS Compared to normal control rats, the erectile function (the ratio of maximal intracavernous pressure to mean arterial blood pressure) was found to be dramatically decreased in diabetic rats. Twenty-eight miRNAs were identified significantly dysregulated in diabetic rat's penile tissues. Among these altered miRNAs, miR-92a expression was dramatically up-regulated in diabetes-induced ED. The expression level of eNOS was significantly lower in diabetic rats' penes than that in normal control rats. Overexpression of miR-92a significantly decreased the expression of eNOS mRNA and protein in rat aortic endothelial cells. On the contrary, inhibition of miR-92a significantly increased the expression of eNOS mRNA and protein in rat aortic endothelial cells. CONCLUSIONS This is the first report demonstrating the altered microRNA expression signature in diabetes-associated ED. In vitro study showed that miR-92a was an important regulator of eNOS expression in endothelial cells, indicating upregulation of miR-92a might play an important role in diabetes-associated deterioration of erectile function. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e421-e422 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jun Yang Wuhan, China, People's Republic of More articles by this author Tao Wang Wuhan, China, People's Republic of More articles by this author Hua Xu Wuhan, China, People's Republic of More articles by this author Shaogang Wang Wuhan, China, People's Republic of More articles by this author Jihong Liu Wuhan, China, People's Republic of More articles by this author Zhangqun Ye Wuhan, China, People's Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...